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Comparison of genotypic and virtual phenotypic drug resistance interpretations with laboratory-based phenotypes among CRF01_AE and subtype B HIV-infected individuals

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ชื่อเรื่อง : Comparison of genotypic and virtual phenotypic drug resistance interpretations with laboratory-based phenotypes among CRF01_AE and subtype B HIV-infected individuals
นักวิจัย : Jiamsakul A. , Chaiwarith R. , Durier N. , Sirivichayakul S. , Kiertiburanakul S. , Van Den Eede P. , Ditangco R. , Kamarulzaman A. , Li P. , Ratanasuwan W. , Sirisanthana T. , Li P. , Lee M. , Kumarasamy N. , Saghayam S. , Pujari S. , Joshi K. , Merati T. , Yuliana F. , Lee C. , Sim B. , Kamarulzaman A. , Ong L. , Mustafa M. , Nordin N. , Ditangco R. , Bantique R. , Chen Y. , Lin Y. , Phanuphak P. , Sirivichayakul S. , Sungkanuparph S. , Kiertiburanakul S. , Chumla L. , Sanmeema N. , Sirisanthana T. , Praparattanapan J. , Kantipong P. , Kambua P. , Ratanasuwan W. , Sriondee R. , Kantor R. , Sohn A. , Durier N. , Singtoroj T. , Cooper D. , Law M. , Boettiger D.
คำค้น : -
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2559
อ้างอิง : 01466615 , 2-s2.0-84954399287 , 10.1002/jmv.24320 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84954399287&origin=inward , http://cmuir.cmu.ac.th/jspui/handle/6653943832/42133
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© 2016 Wiley Periodicals, Inc. HIV drug resistance assessments and interpretations can be obtained from genotyping (GT), virtual phenotyping (VP) and laboratory-based phenotyping (PT). We compared resistance calls obtained from GT and VP with those from PT (GT-PT and VP-PT) among CRF01_AE and subtype B HIV-1 infected patients. GT predictions were obtained from the Stanford HIV database. VP and PT were obtained from Janssen Diagnostics BVBA's vircoType TM HIV-1 and Antivirogram®, respectively. With PT assumed as the "gold standard," the area under the curve (AUC) and the Bland-Altman plot were used to assess the level of agreement in resistance interpretations. A total of 80 CRF01_AE samples from Asia and 100 subtype B from Janssen Diagnostics BVBA's database were analysed. CRF01_AE showed discordances ranging from 3 to 27 samples for GT-PT and 1 to 20 samples for VP-PT. The GT-PT and VP-PT AUCs were 0.76-0.97 and 0.81-0.99, respectively. Subtype B showed 3-61 discordances for GT-PT and 2-75 discordances for VP-PT. The AUCs ranged from 0.55 to 0.95 for GT-PT and 0.55 to 0.97 for VP-PT. Didanosine had the highest proportion of discordances and/or AUC in all comparisons. The patient with the largest didanosine FC difference in each subtype harboured Q151M mutation. Overall, GT and VP predictions for CRF01_AE performed significantly better than subtype B for three NRTIs. Although discrepancies exist, GT and VP resistance interpretations in HIV-1 CRF01_AE strains were highly robust in comparison with the gold-standard PT. © 2015 Wiley Periodicals, Inc.

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