ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Differentiation of mesenchymal stem cells from human amniotic fluid to vascular endothelial cells

หน่วยงาน มหาวิทยาลัยเชียงใหม่

รายละเอียด

ชื่อเรื่อง : Differentiation of mesenchymal stem cells from human amniotic fluid to vascular endothelial cells
นักวิจัย : Tancharoen W. , Aungsuchawan S. , Pothacharoen P. , Markmee R. , Narakornsak S. , Kieodee J. , Boonma N. , Tasuya W.
คำค้น : -
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2560
อ้างอิง : 00651281 , 2-s2.0-85010791401 , 10.1016/j.acthis.2016.11.009 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85010791401&origin=inward , http://cmuir.cmu.ac.th/jspui/handle/6653943832/40664
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

© 2016 Elsevier GmbH Endothelial dysfunction is a principle feature of vascular-related disease. Endothelial cells have been acquired for the purposes of the restoration of damaged tissue in therapeutic angiogenesis. However, their use is limited by expansion capacity and the small amount of cells that are obtained. Human amniotic fluid mesenchymal stem cells (hAF-MSCs) are considered an important source for vascular tissue engineering. In this study, hAF-MSCs were characterized and then induced in order to differentiate into the endothelial-like cells. Human amniotic fluid cells (hAFCs) were obtained from amniocentesis at the second trimester of gestation. The cells were characterized as mesenchymal stem cells by flow cytometry. The results showed that the cells were positive for mesenchymal stem cell markers CD44, CD73, CD90 and HLA-ABC, and negative for CD31, Amniotic fluid stem cells marker: CD117, anti-human fibroblasts, HLA-DR and hematopoietic differentiation markers CD34 and CD45. The hAF-MSCs were differentiated into endothelial cells under the induction of vascular endothelial growth factor (VEGF) and analyzed for the expression of the endothelial-specific markers and function. The expression of the endothelial-specific markers was determined by reverse transcriptase-quantitative PCR (RT-qPCR), while immunofluorescent analysis demonstrated that the induced hAF-MSCs expressed von Willebrand factor (vWF), vascular endothelial growth factor receptor 2 (VEGFR2), CD31 and endothelial nitric oxide synthase (eNOS). The network formation assay showed that the induced hAF-MSCs formed partial networks. All results indicated that hAF-MSCs have the potential to be differentiated into endothelial-like cells, while human amniotic fluid might be a suitable source of MSCs for vascularized tissue engineering.

บรรณานุกรม :
Tancharoen W. , Aungsuchawan S. , Pothacharoen P. , Markmee R. , Narakornsak S. , Kieodee J. , Boonma N. , Tasuya W. . (2560). Differentiation of mesenchymal stem cells from human amniotic fluid to vascular endothelial cells.
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Tancharoen W. , Aungsuchawan S. , Pothacharoen P. , Markmee R. , Narakornsak S. , Kieodee J. , Boonma N. , Tasuya W. . 2560. "Differentiation of mesenchymal stem cells from human amniotic fluid to vascular endothelial cells".
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Tancharoen W. , Aungsuchawan S. , Pothacharoen P. , Markmee R. , Narakornsak S. , Kieodee J. , Boonma N. , Tasuya W. . "Differentiation of mesenchymal stem cells from human amniotic fluid to vascular endothelial cells."
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2560. Print.
Tancharoen W. , Aungsuchawan S. , Pothacharoen P. , Markmee R. , Narakornsak S. , Kieodee J. , Boonma N. , Tasuya W. . Differentiation of mesenchymal stem cells from human amniotic fluid to vascular endothelial cells. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2560.