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Effective enhancement of polylactic acid-degrading enzyme production by Amycolatopsis sp. strain SCM_MK2-4 using statistical and one-factor-at-a-time approaches

หน่วยงาน มหาวิทยาลัยเชียงใหม่

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ชื่อเรื่อง : Effective enhancement of polylactic acid-degrading enzyme production by Amycolatopsis sp. strain SCM_MK2-4 using statistical and one-factor-at-a-time approaches
นักวิจัย : Penkhrue W. , Kanpiengjai A. , Khanongnuch C. , Masaki K. , Pathom-Aree W. , Punyodom W. , Lumyong S.
คำค้น : -
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2560
อ้างอิง : 10826068 , 2-s2.0-85019188426 , 10.1080/10826068.2017.1315597 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85019188426&origin=inward , http://cmuir.cmu.ac.th/jspui/handle/6653943832/40178
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

© 2017 Taylor & Francis. This study aims to find the optimal medium and conditions for polylactic acid (PLA)-degrading enzyme production by Amycolatopsis sp. SCM_MK2-4. Screening of the most effective components in the enzyme production medium by Plackett–Burman design revealed that the silk cocoon and PLA film were the most significant variables enhancing the PLA-degrading enzyme production. After an response surface methodology, a maximum amount of PLA-degrading enzyme activity at 0.74 U mL −1 was predicted and successfully validated at 95% after 0.39% (w/v) silk cocoon and 1.62% (w/v) PLA film were applied to the basal medium. The optimal initial pH value, temperature, and inoculum size were evaluated by a method considering one-factor-at-a-time. The values were recorded at an initial pH in the range of 7.5–9.0, a temperature of 30–32°C, and an inoculum size of 4–10%. The highest activity of approximately 0.95 U mL −1 was achieved after 4 days of cultivation using the optimized medium and under optimized conditions in a shake flask. Upscaling to the use of a 3-L stirred tank fermenter was found to be successful with a PLA-degrading activity of 5.53 U mL −1 ; which represents a 51-fold increase in the activity compared with that obtained from the nonoptimized medium and conditions in the shake flask.

บรรณานุกรม :
Penkhrue W. , Kanpiengjai A. , Khanongnuch C. , Masaki K. , Pathom-Aree W. , Punyodom W. , Lumyong S. . (2560). Effective enhancement of polylactic acid-degrading enzyme production by Amycolatopsis sp. strain SCM_MK2-4 using statistical and one-factor-at-a-time approaches.
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Penkhrue W. , Kanpiengjai A. , Khanongnuch C. , Masaki K. , Pathom-Aree W. , Punyodom W. , Lumyong S. . 2560. "Effective enhancement of polylactic acid-degrading enzyme production by Amycolatopsis sp. strain SCM_MK2-4 using statistical and one-factor-at-a-time approaches".
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Penkhrue W. , Kanpiengjai A. , Khanongnuch C. , Masaki K. , Pathom-Aree W. , Punyodom W. , Lumyong S. . "Effective enhancement of polylactic acid-degrading enzyme production by Amycolatopsis sp. strain SCM_MK2-4 using statistical and one-factor-at-a-time approaches."
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2560. Print.
Penkhrue W. , Kanpiengjai A. , Khanongnuch C. , Masaki K. , Pathom-Aree W. , Punyodom W. , Lumyong S. . Effective enhancement of polylactic acid-degrading enzyme production by Amycolatopsis sp. strain SCM_MK2-4 using statistical and one-factor-at-a-time approaches. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2560.