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Expression patterns of cell adhesion molecules in bovine preimplantation embryos cultured in vitro

หน่วยงาน มหาวิทยาลัยเชียงใหม่

รายละเอียด

ชื่อเรื่อง : Expression patterns of cell adhesion molecules in bovine preimplantation embryos cultured in vitro
นักวิจัย : Sathanawongs,A. , Nganvongpanit,K. , Mekchay,S.
คำค้น : Veterinary (all)
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : 01256491 , 2-s2.0-84884564804 , http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84884564804&origin=inward , http://cmuir.cmu.ac.th/handle/6653943832/39171
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

This study aimed to investigate gene expression patterns throughout preimplantation embryos and their comparative expression in morphological quality blastocysts using real-time RT-PCR. Bovine oocytes underwent maturation process, fertilized and were cultured in vitro. Degree of fragmentation and differentiation were the criteria to categorize day-7 blastocysts as of good and poor quality. All of the embryos from each developmental stage were used to quantify transcript abundance. It was found that E-cadherin was highly expressed in immature and mature oocytes, then it decreased at 2- to 16-cell stages, and later increased in morula and blastocyst stages. The ß-catenin transcript was highly abundant up to the 4-cell stage and further down regulated in the later stages. However, the transcript abundance of desmocollin 2 (DSC2) was high only at morula and blastocyst stages. The relative abundance of E-cadherin, ß-catenin and DSC2 mRNA in good quality blastocysts were statistically significantly higher than in poor quality blastocysts. Moreover, only the protein expression levels of E-cadherin and ß-catenin were significantly higher in good than in poor blastocysts. In conclusion, E-cadherin and ß-catenin are highly expressed in good quality bovine blastocysts. Therefore, they can be used as biomarker for blastocyst quality.

บรรณานุกรม :
Sathanawongs,A. , Nganvongpanit,K. , Mekchay,S. . (2555). Expression patterns of cell adhesion molecules in bovine preimplantation embryos cultured in vitro.
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Sathanawongs,A. , Nganvongpanit,K. , Mekchay,S. . 2555. "Expression patterns of cell adhesion molecules in bovine preimplantation embryos cultured in vitro".
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Sathanawongs,A. , Nganvongpanit,K. , Mekchay,S. . "Expression patterns of cell adhesion molecules in bovine preimplantation embryos cultured in vitro."
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2555. Print.
Sathanawongs,A. , Nganvongpanit,K. , Mekchay,S. . Expression patterns of cell adhesion molecules in bovine preimplantation embryos cultured in vitro. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2555.