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Electrochemically controlled surface plasmon resonance immunosensor for the detection of human immunoglobulin G on poly(3-aminobenzoic acid) ultrathin films

หน่วยงาน มหาวิทยาลัยเชียงใหม่

รายละเอียด

ชื่อเรื่อง : Electrochemically controlled surface plasmon resonance immunosensor for the detection of human immunoglobulin G on poly(3-aminobenzoic acid) ultrathin films
นักวิจัย : Sriwichai S. , Sriwichai S. , Baba A. , Phanichphant S. , Shinbo K. , Kato K. , Kaneko F.
คำค้น : Surfaces, Coatings and Films , Metals and Alloys , Materials Chemistry , Electrical and Electronic Engineering , Electronic, Optical and Magnetic Materials , Instrumentation , Condensed Matter Physics
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2553
อ้างอิง : 09254005 , 2-s2.0-77953137365 , 10.1016/j.snb.2010.03.031 , http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77953137365&origin=inward , http://cmuir.cmu.ac.th/handle/6653943832/38582
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

An electrochemically controlled surface plasmon resonance (SPR) immunosensor based on carboxylated polyaniline was constructed for detecting human immunoglobulin G (IgG) without using further labeled molecules. The carboxylated polyaniline, poly(3-aminobenzoic acid) (PABA), was prepared by electropolymerization of its monomer, 3-aminobenzoic acid (ABA), on a gold-coated high-refractive-index glass slide that was also used as a working electrode for the electrochemical system. The kinetic properties during electropolymerization were monitored in situ with electrochemical-surface plasmon resonance (EC-SPR) spectroscopy. The obtained PABA film, which was used to construct the immunosensor, showed electroactivity in neutral phosphate-buffered saline solution. The carboxylic acid surface of the PABA film was then activated to immobilize anti-human IgG. Finally, the immunosensor electrode was used to probe the binding reaction of anti-human/human IgG with various concentrations of human IgG at different constant applied potentials. The immobilization and binding reaction processes were controlled and also studied in situ by EC-SPR spectroscopy. The binding process at different applied potentials was studied and fitted using the Freundlich model. The surface morphology of the films after the immobilization of anti-human IgG was studied by atomic force microscopy. © 2010 Elsevier B.V. All rights reserved.

บรรณานุกรม :
Sriwichai S. , Sriwichai S. , Baba A. , Phanichphant S. , Shinbo K. , Kato K. , Kaneko F. . (2553). Electrochemically controlled surface plasmon resonance immunosensor for the detection of human immunoglobulin G on poly(3-aminobenzoic acid) ultrathin films.
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Sriwichai S. , Sriwichai S. , Baba A. , Phanichphant S. , Shinbo K. , Kato K. , Kaneko F. . 2553. "Electrochemically controlled surface plasmon resonance immunosensor for the detection of human immunoglobulin G on poly(3-aminobenzoic acid) ultrathin films".
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Sriwichai S. , Sriwichai S. , Baba A. , Phanichphant S. , Shinbo K. , Kato K. , Kaneko F. . "Electrochemically controlled surface plasmon resonance immunosensor for the detection of human immunoglobulin G on poly(3-aminobenzoic acid) ultrathin films."
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2553. Print.
Sriwichai S. , Sriwichai S. , Baba A. , Phanichphant S. , Shinbo K. , Kato K. , Kaneko F. . Electrochemically controlled surface plasmon resonance immunosensor for the detection of human immunoglobulin G on poly(3-aminobenzoic acid) ultrathin films. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2553.