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Divergent targets of Aspergillus fumigatus AcuK and AcuM transcription factors during growth in vitro versus invasive disease

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ชื่อเรื่อง : Divergent targets of Aspergillus fumigatus AcuK and AcuM transcription factors during growth in vitro versus invasive disease
นักวิจัย : Pongpom,M. , Liu,H. , Xu,W. , Snarr,B.D. , Sheppard,D.C. , Mitchell,A.P. , Filler,S.G.
คำค้น : Parasitology , Microbiology , Immunology , Infectious Diseases
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2558
อ้างอิง : 00199567 , 2-s2.0-84923012197 , 10.1128/IAI.02685-14 , http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84923012197&origin=inward , http://cmuir.cmu.ac.th/handle/6653943832/38393
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

© 2015, American Society for Microbiology. In Aspergillus nidulans, the AcuK and AcuM transcription factors form a complex that regulates gluconeogenesis. In Aspergillus fumigatus, AcuM governs gluconeogenesis and iron acquisition in vitro and virulence in immunosuppressed mice. However, the function of AcuK was previously unknown. Through in vitro studies, we found that A. fumigatus ΔacuK single and ΔacuK ΔacuM double mutants had impaired gluconeogenesis and iron acquisition, similar to the ΔacuM mutant. Also, the ΔacuK, ΔacuM, and ΔacuK ΔacuM mutants had similar virulence defects in mice. However, the ΔacuK mutant had a milder defect in extracellular siderophore activity and induction of epithelial cell damage in vitro than did the ΔacuM mutant. Moreover, overexpression of acuM in the ΔacuK mutant altered expression of 3 genes and partially restored growth under iron-limited conditions, suggesting that AcuM can govern some genes independently of AcuK. Although the ΔacuK and ΔacuM mutants had very similar transcriptional profiles in vitro, their transcriptional profiles during murine pulmonary infection differed both from their in vitro profiles and from each other. While AcuK and AcuM governed the expression of only a few iron-responsive genes in vivo, they influenced the expression of other virulence-related genes, such as hexA and dvrA. Therefore, in A. fumigatus, while AcuK and AcuM likely function as part of the same complex, they can also function independently of each other. Furthermore, AcuK and AcuM have different target genes in vivo than in vitro, suggesting that in vivo infection stimulates unique transcriptional regulatory pathways in A. fumigatus.

บรรณานุกรม :
Pongpom,M. , Liu,H. , Xu,W. , Snarr,B.D. , Sheppard,D.C. , Mitchell,A.P. , Filler,S.G. . (2558). Divergent targets of Aspergillus fumigatus AcuK and AcuM transcription factors during growth in vitro versus invasive disease.
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Pongpom,M. , Liu,H. , Xu,W. , Snarr,B.D. , Sheppard,D.C. , Mitchell,A.P. , Filler,S.G. . 2558. "Divergent targets of Aspergillus fumigatus AcuK and AcuM transcription factors during growth in vitro versus invasive disease".
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Pongpom,M. , Liu,H. , Xu,W. , Snarr,B.D. , Sheppard,D.C. , Mitchell,A.P. , Filler,S.G. . "Divergent targets of Aspergillus fumigatus AcuK and AcuM transcription factors during growth in vitro versus invasive disease."
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2558. Print.
Pongpom,M. , Liu,H. , Xu,W. , Snarr,B.D. , Sheppard,D.C. , Mitchell,A.P. , Filler,S.G. . Divergent targets of Aspergillus fumigatus AcuK and AcuM transcription factors during growth in vitro versus invasive disease. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2558.