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Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture

หน่วยงาน มหาวิทยาลัยเชียงใหม่

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ชื่อเรื่อง : Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture
นักวิจัย : Tunma S. , Song D.-H. , Kim S.-E. , Kim K.-N. , Han J.-G. , Boonyawan D.
คำค้น : -
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : 01694332 , 10.1016/j.apsusc.2013.07.046 , ASUSE , http://www.scopus.com/inward/record.url?eid=2-s2.0-84883156237&partnerID=40&md5=e47713846d3d36d4b6205dde23e489f5 , http://cmuir.cmu.ac.th/handle/6653943832/7112
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

In this study, we focused on sericin hydrolysates, originating from silkworm used in serum-free human bone marrow-derived mesenchymal stem cells (hBM-MSCs) culture. We reported the effect of a covalent linkage between a bioactive protein molecule and polystyrene dish surface via a carbon intermediate layer which can slow down the release rate of protein compounds into the phosphate buffer saline (PBS) solution. Films of amorphous carbon (a-C) and functionalized-carbon were deposited on PS culture dish surfaces by using a DC magnetron sputtering system and RF PECVD system. We found that a-C based-films can increase the hydrophilicity and biocompatibility of polystyrene (PS) dishes, especially a-C films and a-C:N2 films showed good attachment of hBM-MSCs at 24 h. However, in the case of silica surface (a-C:SiOx films), the cells showed a ragged and unattached boundary resulting from the presence of surface silanol groups. For the UV-vis absorbance, all carbon modified-PS dishes showed a lower release rate of sericin molecules into PBS solution than PS control. This revealed that the functionalized carbon could be enhanced by specific binding properties with given molecules. The carbon-coated PS dishes grafting with sericin protein were used in a serum-free condition. We also found that hBM-MSCs have higher percentage of proliferated cells at day 7 for the modified dishes with carbon films and coated with sericin than the PS control coated with sericin. The physical film properties were measured by atomic force microscopy (AFM), scanning electron microscope (SEM) and contact angle measurement. The presence of NH2 groups of sericin compounds on the PS dish was revealed by Fourier transform infrared spectroscopy (FTIR). The stability of covalent bonds of sericin molecules after washing out ungrafted sericin was confirmed by X-ray photoelectron spectroscopy (XPS). © 2013 Elsevier B.V. All rights reserved.

บรรณานุกรม :
Tunma S. , Song D.-H. , Kim S.-E. , Kim K.-N. , Han J.-G. , Boonyawan D. . (2556). Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture.
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Tunma S. , Song D.-H. , Kim S.-E. , Kim K.-N. , Han J.-G. , Boonyawan D. . 2556. "Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture".
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Tunma S. , Song D.-H. , Kim S.-E. , Kim K.-N. , Han J.-G. , Boonyawan D. . "Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture."
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2556. Print.
Tunma S. , Song D.-H. , Kim S.-E. , Kim K.-N. , Han J.-G. , Boonyawan D. . Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2556.