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An enzyme-linked immunosorbent assay for detecting 3-phenoxybenzoic acid in plasma and its application to farmers and consumers

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ชื่อเรื่อง : An enzyme-linked immunosorbent assay for detecting 3-phenoxybenzoic acid in plasma and its application to farmers and consumers
นักวิจัย : Thiphom S. , Prapamontol T. , Chantara S. , Mangklabruks A. , Suphavilai C. , Ahn K.C. , Gee S.J. , Hammock B.D.
คำค้น : -
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : 17599660 , 10.1039/c2ay25642h , http://www.scopus.com/inward/record.url?eid=2-s2.0-84868131431&partnerID=40&md5=32f44b4b0e11ee344b6306c764471073 , http://cmuir.cmu.ac.th/handle/6653943832/6960
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The aim of this study was to identify a plasma biomarker of exposure to pyrethroid insecticides. A major metabolite, 3-phenoxybenzoic acid (3-PBA), can be detected in urine but urinary 3-PBA cannot be used to assess the active dose. The 3-PBA-adduct represents a much more persistent class of biomarkers than metabolites excreted into urine, having half-lives of up to several weeks or months. We developed an enzyme-linked immunosorbent assay (ELISA) for total 3-PBA including the adduct formed after alkaline hydrolysis, liquid-liquid extraction (LLE) and solid phase extraction (SPE) of the sample. The developed ELISA had an IC 50 value of 26.7 ng mL -1. The intra- and inter-assay coefficients of variation (%CV) were lower than 5% and were within the optimum condition variance (OCV) range. The LLE cleanup technique satisfactorily eliminated the matrix effect from plasma samples before SPE and ELISA analysis yielding good recoveries (85.9-99.4%) with a limit of quantitation (LOQ, 5 ng mL -1) that was 30- to 47-fold more sensitive than previous studies. Moreover, the method developed could separate more than 80% of 3-PBA from the adducted form. The method was successfully applied for the detection of the target in real samples obtained from consumers (n = 50) and farmers (n = 50). To our knowledge, this is the first ELISA method for detecting 3-PBA in the human plasma that has been applied to a field study. © 2012 The Royal Society of Chemistry.

บรรณานุกรม :
Thiphom S. , Prapamontol T. , Chantara S. , Mangklabruks A. , Suphavilai C. , Ahn K.C. , Gee S.J. , Hammock B.D. . (2555). An enzyme-linked immunosorbent assay for detecting 3-phenoxybenzoic acid in plasma and its application to farmers and consumers.
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Thiphom S. , Prapamontol T. , Chantara S. , Mangklabruks A. , Suphavilai C. , Ahn K.C. , Gee S.J. , Hammock B.D. . 2555. "An enzyme-linked immunosorbent assay for detecting 3-phenoxybenzoic acid in plasma and its application to farmers and consumers".
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Thiphom S. , Prapamontol T. , Chantara S. , Mangklabruks A. , Suphavilai C. , Ahn K.C. , Gee S.J. , Hammock B.D. . "An enzyme-linked immunosorbent assay for detecting 3-phenoxybenzoic acid in plasma and its application to farmers and consumers."
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2555. Print.
Thiphom S. , Prapamontol T. , Chantara S. , Mangklabruks A. , Suphavilai C. , Ahn K.C. , Gee S.J. , Hammock B.D. . An enzyme-linked immunosorbent assay for detecting 3-phenoxybenzoic acid in plasma and its application to farmers and consumers. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2555.