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Simultaneous determination of urinary hydroxylated metabolites of naphthalene, fluorene, phenanthrene, fluoranthene and pyrene as multiple biomarkers of exposure to polycyclic aromatic hydrocarbons

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ชื่อเรื่อง : Simultaneous determination of urinary hydroxylated metabolites of naphthalene, fluorene, phenanthrene, fluoranthene and pyrene as multiple biomarkers of exposure to polycyclic aromatic hydrocarbons
นักวิจัย : Chetiyanukornkul T. , Toriba A. , Kameda T. , Tang N. , Hayakawa K.
คำค้น : -
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2549
อ้างอิง : 16182642 , 10.1007/s00216-006-0628-6 , 16924381 , ABCNB , http://www.scopus.com/inward/record.url?eid=2-s2.0-33749008491&partnerID=40&md5=9fcb02748e0466b8006c7be40d11a600 , http://www.ncbi.nlm.nih.gov/pubmed/16924381 , http://cmuir.cmu.ac.th/handle/6653943832/5074
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

A method is presented for determining monohydroxy polycyclic aromatic hydrocarbons (OHPAHs) having 2-, 3- and 4-rings in human urine by using high-performance liquid chromatography with fluorescence detection. A urine sample containing conjugates of OHPAHs was hydrolysed in the presence of β-glucuronidase/aryl sulfatase and the solution was cleaned up with a solid-phase extraction (C18 and silica). Eight OHPAHs, namely 1- and 2-hydroxynaphthalenes, 2-hydroxyfluorene, 2-, 3- and 4-hydroxyphenanthrenes, 3-hydroxyfluoranthene and 1-hydroxypyrene, were separated and 1- and 9-hydroxyphenanthrenes co-eluted on an alkylamide-type reversed-phase column with fluorimetric detection. The urinary concentrations of OHPAHs were quantified by using deuterated 1-hydoxypyrene as an internal standard. The method showed good repeatability for inter- and intra-day precisions as well as good linearity of calibration curves (r 2 ranged from 0.996 to 0.999). The limits of detection (S/N=3) were in the range from 2.3 fmol to 2.2 pmol per injection. This method was successfully applied to urine samples from non-smoking taxi drivers, traffic policemen and rural villagers of Chiang Mai, Thailand. The results showed higher urinary concentrations of OHPAHs in rural villagers, consistent with higher respiratory exposure to PAHs. © Springer-Verlag 2006.

บรรณานุกรม :
Chetiyanukornkul T. , Toriba A. , Kameda T. , Tang N. , Hayakawa K. . (2549). Simultaneous determination of urinary hydroxylated metabolites of naphthalene, fluorene, phenanthrene, fluoranthene and pyrene as multiple biomarkers of exposure to polycyclic aromatic hydrocarbons.
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Chetiyanukornkul T. , Toriba A. , Kameda T. , Tang N. , Hayakawa K. . 2549. "Simultaneous determination of urinary hydroxylated metabolites of naphthalene, fluorene, phenanthrene, fluoranthene and pyrene as multiple biomarkers of exposure to polycyclic aromatic hydrocarbons".
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Chetiyanukornkul T. , Toriba A. , Kameda T. , Tang N. , Hayakawa K. . "Simultaneous determination of urinary hydroxylated metabolites of naphthalene, fluorene, phenanthrene, fluoranthene and pyrene as multiple biomarkers of exposure to polycyclic aromatic hydrocarbons."
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2549. Print.
Chetiyanukornkul T. , Toriba A. , Kameda T. , Tang N. , Hayakawa K. . Simultaneous determination of urinary hydroxylated metabolites of naphthalene, fluorene, phenanthrene, fluoranthene and pyrene as multiple biomarkers of exposure to polycyclic aromatic hydrocarbons. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2549.