ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Characteristics and anti-proliferative activity of azelaic acid and its derivatives entrapped in bilayer vesicles in cancer cell lines

หน่วยงาน มหาวิทยาลัยเชียงใหม่

รายละเอียด

ชื่อเรื่อง : Characteristics and anti-proliferative activity of azelaic acid and its derivatives entrapped in bilayer vesicles in cancer cell lines
นักวิจัย : Manosroi A. , Panyosak A. , Rojanasakul Y. , Manosroi J.
คำค้น : -
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2550
อ้างอิง : 1061186X , 10.1080/10611860701349315 , 17541842 , JDTAE , http://www.scopus.com/inward/record.url?eid=2-s2.0-34250019352&partnerID=40&md5=6ba25fca717c8dfe55d20c0989167888 , http://www.ncbi.nlm.nih.gov/pubmed/17541842 , http://cmuir.cmu.ac.th/handle/6653943832/4451
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The hydrophilicity and lipophilicity of azelaic acid (AA) were modified to diethyl azelate (DA) which was synthesized by Fisher esterification reaction and identified by IR, MS and 1H NMR and to azelaic acid-β-cyclodextrin complex (AACD) which was prepared by inclusion complexation and identified by IR, DSC and XRD respectively. AA, DA and AACD were entrapped in liposomes and niosomes comprising of l-α-dipalmitoyl phosphatidylcholine (DPPC)/cholesterol at 7:3 molar ratio and Tween61/cholesterol at 1:1 molar ratio, respectively, using a thin-film hydration method with sonication. The size and morphology of these bilayer vesicles were determined by optical and transmission electron microscopy. The particle size was found to be in the range of 90-190 nm. The entrapment efficiency of AA, DA and AACD in all vesicular formulations was more than 80%, as analyzed by HPLC for AA and AACD, and GC for DA. Anti-proliferative activity of AA and its derivatives (DA and AACD) both entrapped and not entrapped in bilayer vesicles, using MTT assay in three cancer cell lines (HeLa, KB and B16F10) comparing with vincristine, were investigated. AACD showed the highest potency comparing to AA in HeLa, KB and B16F10 of 1.48, 1.6 and 1.5 times, respectively. AA entrapped in liposomes was about 90 times more potent than the free AA, and about 1.5 times less potent than vincristine. When entrapped in bilayer vesicles, DA and AACD were more effective than AA in killing cancer cells. AACD entrapped in liposomes gave the highest anti-proliferation activity in HeLa cell lines with the IC50 of 2.3 and 327 times more potent than vincristine and AA, respectively. DA in liposomes demonstrated the IC50 of 0.03 times less potent than vincristine in KB cell lines, while in B16F10 AACD in niosomes showed the IC50 of 0.05 times less potent than vincristine. This study has suggested that the modification of AA by derivatization and complexation as well as the entrapment in bilayer vesicles can enhance its therapeutic efficacy.

บรรณานุกรม :
Manosroi A. , Panyosak A. , Rojanasakul Y. , Manosroi J. . (2550). Characteristics and anti-proliferative activity of azelaic acid and its derivatives entrapped in bilayer vesicles in cancer cell lines.
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Manosroi A. , Panyosak A. , Rojanasakul Y. , Manosroi J. . 2550. "Characteristics and anti-proliferative activity of azelaic acid and its derivatives entrapped in bilayer vesicles in cancer cell lines".
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Manosroi A. , Panyosak A. , Rojanasakul Y. , Manosroi J. . "Characteristics and anti-proliferative activity of azelaic acid and its derivatives entrapped in bilayer vesicles in cancer cell lines."
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2550. Print.
Manosroi A. , Panyosak A. , Rojanasakul Y. , Manosroi J. . Characteristics and anti-proliferative activity of azelaic acid and its derivatives entrapped in bilayer vesicles in cancer cell lines. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2550.