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Highly sulfated hexasaccharide sequences isolated from chondroitin sulfate of shark fin cartilage: Insights into the sugar sequences with bioactivities

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ชื่อเรื่อง : Highly sulfated hexasaccharide sequences isolated from chondroitin sulfate of shark fin cartilage: Insights into the sugar sequences with bioactivities
นักวิจัย : Mizumoto S. , Murakoshi S. , Kalayanamitra K. , Deepa S.S. , Fukui S. , Kongtawelert P. , Yamada S. , Sugahara K.
คำค้น : -
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : 09596658 , 10.1093/glycob/cws137 , 23019154 , GLYCE , http://www.scopus.com/inward/record.url?eid=2-s2.0-84871948536&partnerID=40&md5=5f964749c1cd22c7ace84d124424b7aa , http://cmuir.cmu.ac.th/handle/6653943832/4011
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Chondroitin sulfate (CS) chains regulate the development of the central nervous system in vertebrates and are linear polysaccharides consisting of variously sulfated repeating disaccharides, [-4GlcUAβ1-3GalNAcβ1-] n, where GlcUA and GalNAc represent d-glucuronic acid and N-acetyl-d-galactosamine, respectively. CS chains containing D-disaccharide units [GlcUA(2-O-sulfate)-GalNAc(6-O-sulfate)] are involved in the development of cerebellar Purkinje cells and neurite outgrowth-promoting activity through interaction with a neurotrophic factor, pleiotrophin, resulting in the regulation of signaling. In this study, to obtain further structural information on the CS chains containing d-disaccharide units involved in brain development, oligosaccharides containing D-units were isolated from a shark fin cartilage. Seven novel hexasaccharide sequences, ΔO-D-D, ΔA-D-D, ΔC-D-D, ΔE-A-D, ΔD-D-C, ΔE-D-D and ΔA-B-D, in addition to three previously reported sequences, ΔC-A-D, ΔC-D-C and ΔA-D-A, were isolated from a CS preparation of shark fin cartilage after exhaustive digestion with chondroitinase AC-I, which cannot act on the galactosaminidic linkages bound to D-units. The symbol Δ stands for a 4,5-unsaturated bond of uronic acids, whereas A, B, C, D, E and O represent [GlcUA-GalNAc(4-O- sulfate)], [GlcUA(2-O-sulfate)-GalNAc(4-O-sulfate)], [GlcUA-GalNAc(6-O-sulfate)] , [GlcUA(2-O-sulfate)-GalNAc(6-O-sulfate)], [GlcUA-GalNAc(4-O-, 6-O-sulfate)] and [GlcUA-GalNAc], respectively. In binding studies using an anti-CS monoclonal antibody, MO-225, the epitopes of which are involved in cerebellar development in mammals, novel epitope structures, ΔA-D-A, ΔA-D-D and ΔA-B-D, were revealed. Hexasaccharides containing two consecutive D-units or a B-unit will be useful for the structural and functional analyses of CS chains particularly in the neuroglycobiological fields. © 2012 The Author.

บรรณานุกรม :
Mizumoto S. , Murakoshi S. , Kalayanamitra K. , Deepa S.S. , Fukui S. , Kongtawelert P. , Yamada S. , Sugahara K. . (2556). Highly sulfated hexasaccharide sequences isolated from chondroitin sulfate of shark fin cartilage: Insights into the sugar sequences with bioactivities.
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Mizumoto S. , Murakoshi S. , Kalayanamitra K. , Deepa S.S. , Fukui S. , Kongtawelert P. , Yamada S. , Sugahara K. . 2556. "Highly sulfated hexasaccharide sequences isolated from chondroitin sulfate of shark fin cartilage: Insights into the sugar sequences with bioactivities".
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Mizumoto S. , Murakoshi S. , Kalayanamitra K. , Deepa S.S. , Fukui S. , Kongtawelert P. , Yamada S. , Sugahara K. . "Highly sulfated hexasaccharide sequences isolated from chondroitin sulfate of shark fin cartilage: Insights into the sugar sequences with bioactivities."
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2556. Print.
Mizumoto S. , Murakoshi S. , Kalayanamitra K. , Deepa S.S. , Fukui S. , Kongtawelert P. , Yamada S. , Sugahara K. . Highly sulfated hexasaccharide sequences isolated from chondroitin sulfate of shark fin cartilage: Insights into the sugar sequences with bioactivities. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2556.