ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Cell reservoirs in lymph nodes infected with HIV-1 subtype E differ from subtype B: identification by combined in situ polymerase chain reaction and immunohistochemistry

หน่วยงาน มหาวิทยาลัยเชียงใหม่

รายละเอียด

ชื่อเรื่อง : Cell reservoirs in lymph nodes infected with HIV-1 subtype E differ from subtype B: identification by combined in situ polymerase chain reaction and immunohistochemistry
นักวิจัย : Bhoopat L. , Rithaporn TS. , Khunamornpong S. , Bhoopat T. , Taylor CR. , Thorner PS.
คำค้น : -
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2549
อ้างอิง : 0893-3952 , 16341147 , http://www.ncbi.nlm.nih.gov/pubmed/3502482 , http://cmuir.cmu.ac.th/handle/6653943832/3222
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

In Thailand, the predominant HIV subtype is E, rather than subtype B as in North America and Europe. Subtype E has the ability to replicate in vitro in Langerhans cells. We hypothesized that this cell type might constitute a reservoir for the HIV virus in infected lymph nodes. We examined lymph nodes from 25 HIV-1 subtype E-infected patients to determine the immunophenotype of HIV-1-infected cells, their numbers and their distribution. The presence of HIV was detected either by in situ reverse transcriptase-polymerase chain reaction or immunoperoxidase. Cell identity was determined by double labelling using alkaline phosphatase-based immunohistochemistry. The majority of HIV-infected cells in the lymph nodes were Langerhans cells (CD1a+S100+) and Langerhans-related dendritic cells (p55+S100+). These cells were located in the paracortical areas of lymph nodes, with a few cells scattered at the edges of germinal centers, but were absent from germinal centers themselves, in contrast to the reported distribution of subtype B virus. In addition, multinucleated giant cells were significantly more common in HIV-infected nodes (64%) compared to controls (4%) (P=0.00002). In conclusion, Langerhans histiocytes and related cells are reservoirs for HIV subtype E in lymph nodes. Disrupting the pathway of infection of Langerhans cells and related cells may be a viable strategy to interfere with transmission of HIV subtype E.

บรรณานุกรม :
Bhoopat L. , Rithaporn TS. , Khunamornpong S. , Bhoopat T. , Taylor CR. , Thorner PS. . (2549). Cell reservoirs in lymph nodes infected with HIV-1 subtype E differ from subtype B: identification by combined in situ polymerase chain reaction and immunohistochemistry.
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Bhoopat L. , Rithaporn TS. , Khunamornpong S. , Bhoopat T. , Taylor CR. , Thorner PS. . 2549. "Cell reservoirs in lymph nodes infected with HIV-1 subtype E differ from subtype B: identification by combined in situ polymerase chain reaction and immunohistochemistry".
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Bhoopat L. , Rithaporn TS. , Khunamornpong S. , Bhoopat T. , Taylor CR. , Thorner PS. . "Cell reservoirs in lymph nodes infected with HIV-1 subtype E differ from subtype B: identification by combined in situ polymerase chain reaction and immunohistochemistry."
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2549. Print.
Bhoopat L. , Rithaporn TS. , Khunamornpong S. , Bhoopat T. , Taylor CR. , Thorner PS. . Cell reservoirs in lymph nodes infected with HIV-1 subtype E differ from subtype B: identification by combined in situ polymerase chain reaction and immunohistochemistry. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2549.