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Cytogenetic and molecular evidence for two species in the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand

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ชื่อเรื่อง : Cytogenetic and molecular evidence for two species in the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand
นักวิจัย : Saeung A. , Otsuka Y. , Baimai V. , Somboon P. , Pitasawat B. , Tuetun B. , Junkum A. , Takaoka H. , Choochote W.
คำค้น : -
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2550
อ้างอิง : 09320113 , 10.1007/s00436-007-0645-1 , 17659361 , PARRE , http://www.scopus.com/inward/record.url?eid=2-s2.0-34548584969&partnerID=40&md5=f1efa2dcce48bc0309da66c3a17946e6 , http://www.ncbi.nlm.nih.gov/pubmed/17659361 , http://cmuir.cmu.ac.th/handle/6653943832/2132
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Seventeen isolines of Anopheles barbirostris derived from animal-biting female mosquitoes showed three karyotypic forms: Form A (X2, Y 1) in five isolines from Phetchaburi province; Form B (X1, X3, Y2) in three and eight isolines from Chiang Mai and Ubon Ratchathani provinces, respectively; Form C (X2, Y3) in one isoline from Phetchaburi province. All 17 isolines exhibited an average branch summation of seta 2-VI pupal skins ranging from 12.1-13.0 branches, which was in the limit of A. barbirostris (6-18 branches). Of the 12 human-biting isolines from Chiang Mai province, five isolines showed Form B (X2, Y2), and seven isolines exhibited a new karyotypic form designated as Form E (X2, Y5). All of 12 isolines had an average branch summation of seta 2-VI pupal skins ranging from 22.4-24.5 branches, which was in the limit of Anopheles campestris (17-58 branches). Thus, they were tentatively designated as A. campestris-like Forms B and E. Hybridization between A. campestris-like Forms B and E showed that they were genetically compatible, yielding viable progeny for several generations suggesting conspecific relationships of these two karyotypic forms. Reproductive isolation among crosses between A. campestris-like Form B and A. barbirostris Forms A, B, and C strongly suggested the existence of these two species. In addition, the very low intraspecific variation (genetic distance <0.005) of the nucleotide sequence of ITS2 of the rDNA and COI and COII of mitochondrial DNA of the seven isolines of A. campestris-like Forms B and E supported their conspecific relationship. The large sequence divergence of ITS2 (0.203-0.268), COI (0.026-0.032), and COII (0.030-0.038) from genomic DNA of A. campestris-like Forms B and E and the A. barbirostris Forms A, B, and C clearly supported cytogenetic and morphological evidence. © 2007 Springer-Verlag.

บรรณานุกรม :
Saeung A. , Otsuka Y. , Baimai V. , Somboon P. , Pitasawat B. , Tuetun B. , Junkum A. , Takaoka H. , Choochote W. . (2550). Cytogenetic and molecular evidence for two species in the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand.
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Saeung A. , Otsuka Y. , Baimai V. , Somboon P. , Pitasawat B. , Tuetun B. , Junkum A. , Takaoka H. , Choochote W. . 2550. "Cytogenetic and molecular evidence for two species in the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand".
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Saeung A. , Otsuka Y. , Baimai V. , Somboon P. , Pitasawat B. , Tuetun B. , Junkum A. , Takaoka H. , Choochote W. . "Cytogenetic and molecular evidence for two species in the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand."
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2550. Print.
Saeung A. , Otsuka Y. , Baimai V. , Somboon P. , Pitasawat B. , Tuetun B. , Junkum A. , Takaoka H. , Choochote W. . Cytogenetic and molecular evidence for two species in the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2550.